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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells
doi: 10.3390/ijms21207555
Figure Lengend Snippet: Alpha-smooth muscle actin (α-SMA) and galectin-3 in LX-2 cells expressing chemerin isoforms. ( a – c ) LX-2 cells were transfected with an insertless plasmid (C) and plasmids to express huChem-155, 156 and 157. Cellular α-SMA was analyzed by immunoblot at 24, 48 and 72 h post-transfection. Coomassie stained membrane served as control; ( d – f ) Galectin-3 in the cells described in a–c; ( g – i ) Galectin-3 in the cell media of LX-2 cells described in a–c. N = 3; * p < 0.05, ** p < 0.01, *** p < 0.001, the number in ( h ) in brackets is the p -value.
Article Snippet: The antibodies used were obtained from the following sources: human chemerin, R&D Systems (Wiesbaden-Nordenstadt, Germany); GAPDH, New England Biolabs GmbH (Frankfurt am Main, Germany);
Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot, Staining
Journal: International Journal of Molecular Sciences
Article Title: Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells
doi: 10.3390/ijms21207555
Figure Lengend Snippet: Secretome of HSCs and LX-2 cells. ( a ) IL-6 in the supernatant of HSCs of three different donors and LX-2 cells; ( b ) Pentraxin 3 (PTX3); ( c ) IL-8; ( d ) galectin-3; and ( e ) chemerin in the cell culture media of these cells.
Article Snippet: The antibodies used were obtained from the following sources: human chemerin, R&D Systems (Wiesbaden-Nordenstadt, Germany); GAPDH, New England Biolabs GmbH (Frankfurt am Main, Germany);
Techniques: Cell Culture
Journal: International Journal of Molecular Sciences
Article Title: Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells
doi: 10.3390/ijms21207555
Figure Lengend Snippet: Effect of huChem-157 on IL-6, IL-8, pentraxin 3 (PTX3) and galectin-3 in primary human HSCs of three donors. ( a ) HSCs were incubated with increasing concentrations of recombinant huChem-157 for 24 h and IL-6; ( b ) IL-8; ( c ) PTX3 and ( d ) galectin-3 were measured by ELISA.
Article Snippet: The antibodies used were obtained from the following sources: human chemerin, R&D Systems (Wiesbaden-Nordenstadt, Germany); GAPDH, New England Biolabs GmbH (Frankfurt am Main, Germany);
Techniques: Incubation, Recombinant, Enzyme-linked Immunosorbent Assay